What is the best way to load a gel?

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What is the best way to load a gel?

The best way to load gels is to first make sure the fully solidified gel is submerged in the running buffer with only a thin layer of buffer over the top of the gel. While pipetting their samples into the gel, most researchers guide their pipettor with their other hand to prevent the tip from damaging the gel matrix. Since loading buffer is more dense than running buffer, the pipette tip can be held slightly above the well during filling, and the sample will flow down into the well. Be sure to slowly dispense the sample to avoid sample loss.

2018-05-18T16:51:25+00:00

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