Why does my DNA run slower in one device versus another when all of the experimental parameters (buffer, % agarose, voltage, thickness of gel, etc.) are identical?

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Why does my DNA run slower in one device versus another when all of the experimental parameters (buffer, % agarose, voltage, thickness of gel, etc.) are identical?

The critical factor when comparing two devices is the inter electrode distance. This is the distance (not the length of the gel) that determines field strength. (See question below) The power supply setting is based on this value. For example: to duplicate run conditions of 5volts/cm in two devices with 10 and 12 cm interelectrode distances, the voltage applied must be 50 and 60 volts respectively.

2018-05-18T16:54:00+00:00

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